How DNA probe assay works?

How DELFIA DNA probe assays work

DELFIA DNA probe assays are based on oligonucleotide probes labeled with lanthanide chelates, solid phase capture and measurement by time-resolved fluorometry.

Assays are performed in microtitration format, and the availability of different lanthanides (Eu, Tb, Sm, and Dy) enable multiplexing up to quadruple analysis in a single assay well.


A typical DELFIA DNA probe assay is the research assays for investigation of genes involved in predisposition to celiac disease, shown below. In this assay three different labels are used and two wells are required for each determination.